The Division I regional football alignments for the 2015 and 2016 season were approved by the TSSAA Board of Control on November 13, 2014.
| School | City | ||
| Class 1A | Region 1 | Cloudland High School | Roan Mountain |
| Concord Christian | Knoxville | ||
| Hancock Co. High School | Sneedville | ||
| Oakdale High School | Oakdale | ||
| Unaka High School | Elizabethton | ||
| Region 2 | Coalfield High School | Coalfield | |
| Greenback High School | Greenback | ||
| Harriman High School | Harriman | ||
| Jellico High School | Jellico | ||
| Midway High School | Kingston | ||
| Oneida High School | Oneida | ||
| Sunbright High School | Sunbright | ||
| Region 3 | Copper Basin High School | Copperhill | |
| Grace Baptist Academy | Chattanooga | ||
| Lookout Valley High School | Chattanooga | ||
| Sale Creek School | Sale Creek | ||
| South Pittsburg High School | South Pittsburg | ||
| Whitwell High School | Whitwell | ||
| Region 4 | Columbia Academy | Columbia | |
| Cornersville High School | Cornersville | ||
| Fayetteville High School | Fayetteville | ||
| Grace Christian Academy | Franklin | ||
| Huntland High School | Huntland | ||
| Moore Co. High School | Lynchburg | ||
| Mt. Pleasant High School | Mt. Pleasant | ||
| Richland High School | Lynnville | ||
| Summertown High School | Summertown | ||
| Region 5 | Clay Co. High School | Celina | |
| Gordonsville High School | Gordonsville | ||
| Jo Byrns High School | Cedar Hill | ||
| Middle Tennessee Christian School | Murfreesboro | ||
| Monterey High School | Monterey | ||
| Nashville Christian School | Nashville | ||
| Pickett Co. High School | Byrdstown | ||
| Red Boiling Springs High School | Red Boiling Springs | ||
| Trousdale Co. High School | Hartsville | ||
| Region 6 | Clarksville Academy | Clarksville | |
| Collinwood High School | Collinwood | ||
| Hollow Rock-Bruceton Central High School | Bruceton | ||
| Houston Co. High School | Erin | ||
| Huntingdon High School | Huntingdon | ||
| McEwen High School | Mcewen | ||
| Perry Co. High School | Linden | ||
| Wayne Co. High School | Waynesboro | ||
| Region 7 | Dresden High School | Dresden | |
| Gleason High School | Gleason | ||
| Greenfield High School | Greenfield | ||
| Humboldt High School | Humboldt | ||
| Lake Co. High School | Tiptonville | ||
| Peabody High School | Trenton | ||
| South Fulton High School | South Fulton | ||
| Union City High School | Union City | ||
| West Carroll High School | Atwood | ||
| Region 8 | B. T. Washington High School | Memphis | |
| George Washington Carver High School | Memphis | ||
| KIPP Memphis Collegiate School | Memphis | ||
| Memphis Middle College High School | Memphis | ||
| Memphis Northside High School | Memphis | ||
| Middleton High School | Middleton | ||
| W.E.B. Dubois High School | Memphis | ||
| Woodstock High School | Memphis | ||
| Class 2A | Region 1 | Cosby High School | Cosby |
| Cumberland Gap High School | Cumberland Gap | ||
| Gatlinburg-Pittman High School | Gatlinburg | ||
| Hampton High School | Hampton | ||
| Happy Valley High School | Elizabethton | ||
| North Greene High School | Greeneville | ||
| South Greene High School | Greeneville | ||
| Sullivan North High School | Kingsport | ||
| Region 2 | Austin-East High School | Knoxville | |
| Grace Christian Academy | Knoxville | ||
| Oliver Springs High School | Oliver Springs | ||
| Rockwood High School | Rockwood | ||
| Sweetwater High School | Sweetwater | ||
| Tellico Plains High School | Tellico Plains | ||
| Wartburg Central High School | Wartburg | ||
| Region 3 | Bledsoe Co. High School | Pikeville | |
| Boyd Buchanan High School | Chattanooga | ||
| Brainerd High School | Chattanooga | ||
| Marion Co. High School | Jasper | ||
| Meigs Co. High School | Decatur | ||
| Polk Co. High School | Benton | ||
| Silverdale Baptist Academy | Chattanooga | ||
| Tyner Academy | Chattanooga | ||
| Region 4 | Cascade High School | Wartrace | |
| Community High School | Unionville | ||
| Eagleville High School | Eagleville | ||
| East Robertson High School | Cross Plains | ||
| Forrest High School | Chapel Hill | ||
| Jackson Co. High School | Gainesboro | ||
| Watertown High School | Watertown | ||
| Westmoreland High School | Westmoreland | ||
| Region 5 | Decatur Co. Riverside High School | Decaturville | |
| Hickman Co. High School | Centerville | ||
| Lewis Co. High School | Hohenwald | ||
| Loretto High School | Loretto | ||
| Scotts Hill High School | Reagan | ||
| Waverly Central High School | Waverly | ||
| Region 6 | Adamsville High School | Adamsville | |
| Gibson Co. High School | Dyer | ||
| Halls High School | Halls | ||
| Jackson Christian School | Jackson | ||
| McKenzie High School | Mckenzie | ||
| Trinity Christian Academy | Jackson | ||
| Region 7 | Frederick Douglass High School | Memphis | |
| Hillcrest High School | Memphis | ||
| Martin Luther King, Jr. Preparatory High School | Memphis | ||
| Memphis Academy of Health Sciences | Memphis | ||
| Trezevant High School | Memphis | ||
| Region 8 | Fairley High School | Memphis | |
| Manassas High School | Memphis | ||
| Mitchell High School | Memphis | ||
| Oakhaven High School | Memphis | ||
| Westwood High School | Memphis | ||
| Class 3A | Region 1 | Chuckey-Doak High School | Afton |
| Claiborne High School | New Tazewell | ||
| Elizabethton High School | Elizabethton | ||
| Johnson Co. High School | Mountain City | ||
| Northview Academy | Kodak | ||
| Pigeon Forge High School | Pigeon Forge | ||
| Unicoi Co. High School | Erwin | ||
| West Greene High School | Mosheim | ||
| Region 2 | Alcoa High School | Alcoa | |
| Christian Academy of Knoxville | Knoxville | ||
| Kingston High School | Kingston | ||
| Loudon High School | Loudon | ||
| McMinn Central High School | Englewood | ||
| Scott High School | Huntsville | ||
| Region 3 | Chattanooga Christian School | Chattanooga | |
| East Ridge High School | Chattanooga | ||
| Notre Dame High School | Chattanooga | ||
| Red Bank High School | Chattanooga | ||
| Signal Mountain High School | Signal Mountain | ||
| The Howard School | Chattanooga | ||
| Region 4 | Alvin C. York Institute | Jamestown | |
| Cannon Co. High School | Woodbury | ||
| DeKalb Co. High School | Smithville | ||
| Grundy Co. High School | Coalmont | ||
| Sequatchie Co. High School | Dunlap | ||
| Smith Co. High School | Carthage | ||
| Upperman High School | Baxter | ||
| Region 5 | Christ Presbyterian Academy | Nashville | |
| East Nashville Magnet School | Nashville | ||
| Goodpasture Christian School | Madison | ||
| Greenbrier High School | Greenbrier | ||
| Stratford High School | Nashville | ||
| White House Heritage High School | White House | ||
| Whites Creek High School | Whites Creek | ||
| Region 6 | Camden Central High School | Camden | |
| Cheatham Co. Central High School | Ashland City | ||
| East Hickman High School | Lyles | ||
| Fairview High School | Fairview | ||
| Harpeth High School | Kingston Springs | ||
| Stewart Co. High School | Dover | ||
| Sycamore High School | Pleasant View | ||
| Region 7 | Dyersburg High School | Dyersburg | |
| Jackson Central-Merry Academy of Medical Tech. | Jackson | ||
| Jackson South Side High School | Jackson | ||
| Liberty Technology Magnet High School | Jackson | ||
| McNairy Central High School | Selmer | ||
| Milan High School | Milan | ||
| South Gibson Co. High School | Medina | ||
| Westview High School | Martin | ||
| Region 8 | Bolivar Central High School | Bolivar | |
| Covington High School | Covington | ||
| Fayette-Ware Comprehensive High School | Somerville | ||
| Hamilton High School | Memphis | ||
| Melrose High School | Memphis | ||
| Raleigh Egypt High School | Memphis | ||
| Sheffield High School | Memphis | ||
| Class 4A | Region 1 | Cherokee High School | Rogersville |
| Grainger High School | Rutledge | ||
| Greeneville High School | Greeneville | ||
| Seymour High School | Seymour | ||
| Sullivan Central High School | Blountville | ||
| Sullivan East High School | Bluff City | ||
| Sullivan South High School | Kingsport | ||
| Volunteer High School | Church Hill | ||
| Region 2 | Anderson Co. High School | Clinton | |
| Carter High School | Strawberry Plains | ||
| Fulton High School | Knoxville | ||
| Knoxville Catholic High School | Knoxville | ||
| Knoxville Central High School | Knoxville | ||
| Union Co. High School | Maynardville | ||
| Region 3 | Chattanooga Central High School | Harrison | |
| Cumberland Co. High School | Crossville | ||
| East Hamilton High School | Ooltewah | ||
| Hixson High School | Hixson | ||
| Livingston Academy | Livingston | ||
| Sequoyah High School - Madisonville | Madisonville | ||
| Stone Memorial High School | Crossville | ||
| Region 4 | Giles Co. High School | Pulaski | |
| Lawrence Co. High School | Lawrenceburg | ||
| Marshall Co. High School | Lewisburg | ||
| Page High School | Franklin | ||
| Spring Hill High School | Columbia | ||
| Tullahoma High School | Tullahoma | ||
| Region 5 | Macon Co. High School | Lafayette | |
| Maplewood Comprehensive High School | Nashville | ||
| Pearl Cohn High School | Nashville | ||
| Portland High School | Portland | ||
| Springfield High School | Springfield | ||
| White House High School | White House | ||
| Region 6 | Creek Wood High School | Charlotte | |
| Hillwood High School | Nashville | ||
| Kenwood High School | Clarksville | ||
| Lipscomb Academy | Nashville | ||
| Montgomery Central High School | Cunningham | ||
| Northwest High School | Clarksville | ||
| Region 7 | Chester Co. High School | Henderson | |
| Crockett Co. High School | Alamo | ||
| Dyer Co. High School | Newbern | ||
| Hardin Co. High School | Savannah | ||
| Haywood High School | Brownsville | ||
| Jackson North Side High School | Jackson | ||
| Lexington High School | Lexington | ||
| Obion Co. Central High School | Troy | ||
| Ripley High School | Ripley | ||
| Region 8 | Craigmont High School | Memphis | |
| Kingsbury High School | Memphis | ||
| Memphis East High School | Memphis | ||
| Millington Central High School | Millington | ||
| Ridgeway High School | Memphis | ||
| Class 5A | Region 1 | Cocke Co. High School | Newport |
| Daniel Boone High School | Gray | ||
| David Crockett High School | Jonesborough | ||
| Morristown-Hamblen High School East | Morristown | ||
| Morristown-Hamblen High School West | Morristown | ||
| Sevier Co. High School | Sevierville | ||
| Tennessee High School | Bristol | ||
| Region 2 | Gibbs High School | Corryton | |
| Heritage High School | Maryville | ||
| Knoxville Halls High School | Knoxville | ||
| Powell High School | Powell | ||
| South Doyle High School | Knoxville | ||
| West High School | Knoxville | ||
| Region 3 | Campbell Co. High School | Jacksboro | |
| Clinton High School | Clinton | ||
| Farragut High School | Knoxville | ||
| Karns High School | Knoxville | ||
| Lenoir City High School | Lenoir City | ||
| Oak Ridge High School | Oak Ridge | ||
| Region 4 | Cleveland High School | Cleveland | |
| McMinn Co. High School | Athens | ||
| Ooltewah High School | Ooltewah | ||
| Rhea County High School | Evensville | ||
| Soddy-Daisy High School | Soddy-daisy | ||
| Walker Valley High School | Cleveland | ||
| White Co. High School | Sparta | ||
| Region 5 | Columbia Central High School | Columbia | |
| Franklin Co. High School | Winchester | ||
| Independence High School | Thompson''s Station | ||
| LaVergne High School | Lavergne | ||
| Lincoln Co. High School | Fayetteville | ||
| Shelbyville Central High School | Shelbyville | ||
| Stewarts Creek High School | Smyrna | ||
| Summit High School | Spring Hill | ||
| Region 6 | Beech Senior High School | Hendersonville | |
| Cane Ridge High School | Antioch | ||
| Gallatin High School | Gallatin | ||
| Glencliff High School | Nashville | ||
| Hendersonville High School | Hendersonville | ||
| Hillsboro High School | Nashville | ||
| Hunters Lane High School | Nashville | ||
| Station Camp High School | Gallatin | ||
| Region 7 | Brentwood High School | Brentwood | |
| Centennial High School | Franklin | ||
| Clarksville High School | Clarksville | ||
| Dickson Co. High School | Dickson | ||
| Henry Co. High School | Paris | ||
| Northeast High School | Clarksville | ||
| Rossview High School | Clarksville | ||
| West Creek High School | Clarksville | ||
| Region 8 | Bolton High School | Arlington | |
| Brighton High School | Brighton | ||
| Kirby High School | Memphis | ||
| Memphis Central High School | Memphis | ||
| Munford High School | Munford | ||
| Overton High School | Memphis | ||
| Southwind High School | Memphis | ||
| Wooddale High School | Memphis | ||
| Class 6A | Region 1 | Bearden High School | Knoxville |
| Bradley Central High School | Cleveland | ||
| Dobyns Bennett High School | Kingsport | ||
| Hardin Valley Academy | Knoxville | ||
| Jefferson Co. High School | Dandridge | ||
| Maryville High School | Maryville | ||
| Science Hill High School | Johnson City | ||
| William Blount High School | Maryville | ||
| Region 2 | Blackman High School | Murfreesboro | |
| Coffee Co. Central High School | Manchester | ||
| Cookeville High School | Cookeville | ||
| Oakland High School | Murfreesboro | ||
| Riverdale High School | Murfreesboro | ||
| Siegel High School | Murfreesboro | ||
| Smyrna High School | Smyrna | ||
| Warren Co. High School | Mcminnville | ||
| Region 3 | Antioch High School | Antioch | |
| Franklin High School | Franklin | ||
| John Overton High School | Nashville | ||
| Lebanon High School | Lebanon | ||
| McGavock High School | Nashville | ||
| Mt. Juliet High School | Mt. Juliet | ||
| Ravenwood High School | Brentwood | ||
| Wilson Central High School | Lebanon | ||
| Region 4 | Arlington High School | Arlington | |
| Bartlett High School | Bartlett | ||
| Collierville High School | Collierville | ||
| Cordova High School | Cordova | ||
| Germantown High School | Germantown | ||
| Houston High School | Germantown | ||
| White Station High School | Memphis | ||
| Whitehaven High School | Memphis |
