2000 TSSAA Division I Class A Softball Region/Sectional Tournament Bracket
| Seeding | May 15 Regional Semi-Finals |
May 17 Regional Finals |
May 19 Sectional |
May 23-27 State | |||
| (H) Dist. 1 W | Unaka 17 | ||||||
| ¦ | Unaka 8 | ||||||
| Dist. 2 R-Up | Wasburn 0 | ¦ | ¦ | ||||
| ¦ | Unaka 3 | ||||||
| (H) Dist. 2 W | Jellico 6 | ¦ | ¦ | ||||
| ¦ | North Greene 3 | ¦ | ¦ | Unaka | |||
| Dist. 1 R-Up | North Greene 14 | ¦ | ¦ | ||||
| Oneida 2 | ¦ | ||||||
| (H) Dist. 3 W | Cosby 3 | ||||||
| ¦ | Cosby 11 | ||||||
| Dist. 4 R-Up | Oakdale 0 | ¦ | ¦ | ||||
| ¦ | Cosby 5 | ||||||
| (H) Dist. 4 W | Oneida 8 | ¦ | ¦ | ||||
| ¦ | Oneida | ¦ | ¦ | Cosby | |||
| Dist. 3 R-Up | Midway 7 | ¦ | ¦ | ||||
| North Greene 4 | ¦ | ||||||
| (H) Dist. 5 W | Boyd Buchanan 2 | ||||||
| ¦ | Boyd Buchanan 5 | ||||||
| Dist. 6 R-Up | Lookout Valley 0 | ¦ | ¦ | ||||
| ¦ | Boyd Buchanan 10 | ||||||
| (H) Dist. 6 W | Chatt. Christian | ¦ | ¦ | ||||
| ¦ | Grace Aca. 2 | ¦ | ¦ | Boyd Buchanan | |||
| Dist. 5 R-Up | Grace Aca. | ¦ | ¦ | ||||
| Red Boiling Spr. 0 | ¦ | ||||||
| (H) Dist. 7 W | Red Boiling Spr. 2 | ||||||
| ¦ | Red Boiling Spr. 0 | ||||||
| Dist. 8 R-Up | Friendship Chr. 1 | ¦ | ¦ | ||||
| ¦ | Trousdale Co. 5 | ||||||
| (H) Dist. 8 W | Trousdale Co. 11 | ¦ | ¦ | ||||
| ¦ | Trousdale Co. | ¦ | ¦ | Trousdale Co. | |||
| Dist. 7 R-Up | Celina 0 | ¦ | ¦ | ||||
| Grace Aca. 4 | ¦ | ||||||
| (H) Dist. 9 W | Ezell Harding 6 | ||||||
| ¦ | Ezell Harding 5 | ||||||
| Dist. 10 R-Up | McEwen 0 | ¦ | ¦ | ||||
| ¦ | Ezell Harding 8 | ||||||
| (H) Dist. 10 W | Davidson Aca. 3 | ¦ | ¦ | ||||
| ¦ | Davidson Aca. 0 | ¦ | ¦ | Ezell Harding | |||
| Dist. 9 R-Up | Franklin Road Aca. 1 | ¦ | ¦ | ||||
| Summertown 0 | ¦ | ||||||
| (H) Dist. 11 W | Forrest | ||||||
| ¦ | Columbia Aca. 14 | ||||||
| Dist. 12 R-Up | Columbia Aca. | ¦ | ¦ | ||||
| ¦ | Columbia Aca. 1 | ||||||
| (H) Dist. 12 W | Summertown 4 | ¦ | ¦ | ||||
| ¦ | Summertown 0 | ¦ | ¦ | Davidson Aca. | |||
| Dist. 11 R-Up | Moore Co. 3 | ¦ | ¦ | ||||
| Davidson Aca. 3 | ¦ | ||||||
| (H) Dist. 13 W | Huntindgon | ||||||
| ¦ | USJ 2 | ||||||
| Dist. 14 R-Up | Univ. Sch. Jackson | ¦ | ¦ | ||||
| ¦ | USJ 7 | ||||||
| (H) Dist. 14 W | Adamsville 15 | ¦ | ¦ | ||||
| ¦ | Adamsville 0 | ¦ | ¦ | Univ. Sch. of Jackson | |||
| Dist. 13 R-Up | Bruceton 3 | ¦ | ¦ | ||||
| Trenton 0 | ¦ | ||||||
| (H) Dist. 15 W | South Fulton 5 | ||||||
| ¦ | South Fulton 8 | ||||||
| Dist. 16 R-Up | Halls 0 | ¦ | ¦ | ||||
| ¦ | South Fulton 3 | ||||||
| (H) Dist. 16 W | Trenton 9 | ¦ | ¦ | ||||
| ¦ | Trenton 0 | ¦ | ¦ | South Fulton | |||
| Dist. 15 R-Up | Gleason 0 | ¦ | ¦ | ||||
| Adamsville 0 | ¦ |
If a district winner from a district that is to host the regional finals is defeated, the district runner-up from that district shall host the regional finals. (Example: If it is District 2's time to host the region and the District 2 winner is defeated, the District 2 runner-up (provided that team wins) shall host the regional finals. If both teams from District 2 are defeated in the regional semi-final games, the District 1 winner shall host the regional finals.)